Differential immune response of influenza A virus-infected dendritic cells and association with autophagy

We sought to study the responses of dendritic cells (DCs) after direct stimulation by different influenza A viruses.

 Jing Li1, Jiao Lu1,2, Shuang Zhang1, Jing Wang1,2, HaoyuWang1,3, Fei Liu1, MinFang1, Xuefeng Duan*,1 & Wenjun Liu1

1 CAS Key Laboratory of Pathogenic Microbiology & Immunology, Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang District, Beijing, 100101, China
2 University of Chinese Academy of Sciences, Beijing 100101, China
3 School of Life Sciences, Anhui University, Hefei 230601, China
*  Author for correspondence: duanxf@im.ac.cn
PubYear : 2017
Volume : online
Publication Name: Research Article
Page number :
Aim: We sought to study the responses of dendritic cells (DCs) after direct stimulation by different influenza A viruses. Materials & methods: Using bone marrow-derived DCs (BMDCs) as a model, we measured the expression of surface markers, cytokine production and the priming effect on CD4+ naive T cells. Results & conclusion: We found that all of the tested viruses induced BMDC maturation. Cytokine expression assays also demonstrated that activated BMDCs secrete higher levels of cytokines. Similar to the maturation degree, well-stimulated BMDCs induced higher levels of naive CD4+ T-cell activation. Furthermore, we found that the PR8 andWSN influenza A viruse-induced BMDC functional activation was at least partially influenced by autophagy.
First draft submitted: 14 April 2017; Accepted for publication: 16 August 2017; Published online:18 October 2017
Keywords :autophagy . bone marrow-derived DC activation . CD4+ T cells. cytokine production. dendritic cells. immune response. infectious diseases. influenza A virus. RNA-seq analysis
 
This work was supported by grants from the National Key R&D Program of China (2016YFD0500206, 2016YFC1200803), National Natural Science Foundation of China (NSFC; grant no. 31572526, 31402216, 31630079), the Southeast Asia Biodiversity Research Institute, Chinese Academy of Science (Y4ZK111B01).